With this initial introductory offer, you get to try out the Lumin set of your choice for $3 shipped. I do the cycle in the morning before work and I get home and have a shower and I still have a smooth face and not dry and yuk – thank you Lumin, you saved my face and my youth. #LUMIN PRODUCTS SKIN#Ok so for years I have battled with my skin due to my job, I’m a plasterer and always around dust, it’s very bad for your skin and drys it out, but now I’m a 40-year-old and feel awesome waking up and even going to bed. I have loved my experience with the website, subscription, and email services I have received. Each product is beautiful in consistency and fantastic quality. Charcoal cleanser, moisturizing balm, and exfoliator. I have had lots of comments from people around me saying how great my skin is looking. I am so impressed with the results I have been seeing. #LUMIN PRODUCTS FREE#Please feel free to hit me up with any follow up questions.I have been using Lumin Skincare for over two months now. If I had to cherry pick a few to try initially, I would suggest the face wash, scalp treatment, and moisturizer. What works for me, might not work for you, and vice versa. It’s important to remember that skincare products are highly personal. I’m so thankful they let me try so many out. Overall, I was very pleased with the performance of Lumin’s skincare and grooming products. Not my something I would recommend using. I found it difficult to lather, and it seemed generally thin to me. Unfortunately, the same could not be said for the body wash. My scalp felt cleaner and I honestly think it helped my hair feel better too. This was certainly not the case with Lumin’s shampoo and conditioner.įor me, the best product I tried in this collection was the scalp treatment. Some cleansers will remove all the good oils your hair needs too for the sake of “cleaning it”. More importantly, my hair did not feel stripped or dry at all. My hair felt softer and cleaner after using them for just a few days. The shampoo and conditioner were excellent. Now let’s move on to the hair and body products from Lumin. Lumin Grooming Products Review Lumin Skincare and Grooming Products Review I found this scrub to be thinner and more mild than I prefer. Since I use them less frequently, I want a scrub that gets in there and exfoliates. I personally prefer scrubs that are thicker and have some abrasiveness. I think the face scrub was my least favorite product I tried. So if you want to avoid sliming up your pillow case, go light and add more if necessary. I’ve tried a good number of eye products over the years, and I think this one did a great job of brightening my eyes the next morning. I absolutely noticed a brightening of the skin around my eyes using this product. I’ve always had concerns about dark circles around my eyes, and that’s only been compounded by lack of sleep from stress while quarantining and homeschooling. This moisturizer is a great way to get in the habit. Unfortunately, I think too many men neglect this step. Moisturizing is an important part of any skincare regimen. After I’ve applied it, my skin feels supple, but, at the same time, protected. I think it is quite suitable for almost all skin types, but I think adding something with salicylic acid during the week wouldn’t be a bad idea. No nights where you say you’re too tired to wash your face. What I found was that this cleanser really works best if you commit to it. The Lumin charcoal cleanser is stronger than others I have tried in the past and I did have better results. They clean well, but I have found my skin responds better to salicylic acid. I have reviewed charcoal face washes in the past, and have not always had the best of luck with them. Lumin Skincare Products Review Lumin Skincare and Grooming Products Reviewįirst and foremost, we have to talk about the charcoal face wash. First, I want to talk about the skincare portion of Lumin’s line, and then I’ll get into the grooming products. I’ve decided to divide this review up into two parts. Recently the good folks at Lumin sent me some samples of their grooming products to try out and see if they could actually live up to the task. Lumin Skincare is looking to walk the tightrope of the middle ground and offer a face wash that cleans deep and into pores without stripping all of my natural skin oils. Too many cleansers either strip away every so that all I’m left with is a face that feels like I just stared at the open ark of the covenant or they are too gentle which leads to breakouts which I’m trying to avoid in the first place. Heres’ my problem with having combination skin.
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With a very high specificity and sensitivity (up to 99%) and can be very useful to analyze WGSĭata of bacterial genomes and their antimicrobial resistance genes. transposases or because k-mer content or marker genes cannot be easily identified.Ĭonclusion: The newly developed tool is able to determine if contigs are chromosomal or plasmid Prediction of small contigs remains unreliable, since these contigs consists primarily of repeated sequences present in both plasmid and chromosome, e.g. Single copy chromosomal marker genes, plasmid genes, k-mer content and length of contig all appear to be informative, however k-mer content is highly specific for taxa. Results: We show that RFplasmid is able to predict chromosomal and plasmid contigs with error rates ranging from 0.002% to 4.66% and that the use of taxon specific models can be superior to a general plasmid prediction model. On separate test sets of known chromosomal and plasmid contigs of the different bacteria. A prediction model was trained using Random Forest onĪn extensive set of plasmids and chromosomes from 19 different bacterial species and validated Methods: The tool identifies the number of chromosomal marker genes, plasmid replication genesĪnd plasmid typing genes using CheckM and DIAMOND Blast, and determines pentamerįrequencies and contig sizes per contig. coli, and Salmonella, and can also be used for metagenomic The tool is optimized for 19 different bacterial species, This information can be used to determine if a resistant gene isĬhromosomally located or on a plasmid. With our newlyĭeveloped prediction tool, we analyze the composition of these contigs to predict their likely Genome sequence assembly generally results in 50-300 DNA fragments (contigs). However, it is not easy to determine if the gene is located on the chromosome or on a plasmid as Sequence (WGS) analysis makes it easy to determine if a strain contains a resistance gene, These plasmids can spread the AMR genes between bacteria, it is important to know if the genesĪre located on highly transferable plasmids or in the more stable chromosomes. Introduction: Antimicrobial resistant (AMR) genes in bacteria are often carried on plasmids. Linda van der Graaf-van Bloois, Jaap Wagenaar, Aldert Zomer Predicting plasmid contigs from assemblies using single copy marker genes, plasmid genes, kmers Assemble a plasmid macvector install#Systemwide install of development version from Github.Installing development version from Github as regular user.Published by Cold Spring Harbor Laboratory Press.Predicting plasmid contigs from assemblies WebinterfaceĪ web-interface to test single fasta files is available here: Table of Contents Our analysis revealed the extreme variability of plasmids and has led to the discovery of many novel plasmids (including many plasmids carrying antibiotic-resistance genes) without significant similarities to currently known ones. We assembled plasmids in diverse data sets and have shown that thousands of plasmids remained below the radar in already completed genomic and metagenomic studies. We present the metaplasmidSPAdes tool for plasmid assembly in metagenomic data sets that reduced the false positive rate of plasmid detection compared with the state-of-the-art approaches. The recently developed plasmidSPAdes assembler addressed some of these challenges in the case of isolate genomes but stopped short of detecting plasmids in metagenomic assemblies, an untapped source of yet to be discovered plasmids. Although plasmids are important for bacterial survival and adaptation, plasmid detection and assembly from genomic, let alone metagenomic, samples remain challenging.
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